
Proteomics & Metabolomics
Contacts
General Contact
860 486 3319
Staff

Jeremy Balsbaugh, Ph.D.
Facility Director
- Office: Pharmacy/Biology Building Room 531
- Lab: Pharmacy/Biology Building Rooms 511/513
- jeremy.balsbaugh@uconn.edu
- 860.486.3319

Jen Liddle, Ph.D.
Facility Scientist
- Office: Pharmacy/Biology Building Room 513
- Lab: Pharmacy/Biology Building Rooms 511/513
- jennifer.liddle@uconn.edu
- 860.486.1591
Faculty Scientific Advisers

Spencer Nyholm, Ph.D.
Associate Professor
- Biology Physics Building 405
- spencer.nyholm@uconn.edu
- 860.486.4886

Daniel Schwartz, Ph.D.
Associate Professor
- Pharmacy Biology Rm 217/219
- daniel.schwartz@uconn.edu
- 860.486.0596

Xudong Yao, Ph.D.
Associate Professor
- Chemistry A-419
- x.yao@uconn.edu
- 860.486.6644

David Grant
Professor
- Pharmacy 532
- david.grant@uconn.edu
- 860.486.4265
Location
Campus Address
Pharmacy/Biology Building
69 North Eagleville Road, Rooms 511/513
Storrs, CT 06269
Mailing Address
75 North Eagleville Road
TLS Room 175
Storrs, CT 06269
Instrumentation

Sample Preparation

Thermo Scientific Q Exactive HF Mass Spectrometer
- Provides high resolution, accurate mass determinations for MS and MS/MS analysis of proteins and peptides in complex mixtures using High-Field (HF) Orbitrap technology
- Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
- Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity

Available Software
- Proteomics: MaxQuant (Ref), Proteome Discoverer v2.5 with XlinkX node (Thermo Scientific), Byonic (Protein Metrics, Inc.), Scaffold v5.0 (Proteome Software, Inc.)
- Metabolomics: MassLynx (Waters) and Progenesis QI (Nonlinear Dynamics)

Waters Synapt G2-Si Mass Spectrometer
- Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technology
- Capable of multiple mass spectral acquisition options including DDA and DIA modes (MSe)
- Ion mobility technology provides another dimension of separation based on collisional-cross section (analyte size and shape) when mass and retention time aren’t sufficient to separate chemically ambiguous analytes and/or isomers
- Directly coupled to a Waters Acquity UPLC equipped with a PDA detector for high resolution liquid-phase separations at micro-flow rates (μL/min)

Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer
- Provides multiple mass analysis and detection modes for MS and MS/MS using an Orbitrap or dual-pressure linear ion trap
- Features multiple types of gas-phase fragmentation including collision-induced dissociation (CID), higher energy C-trap dissociation (HCD), electron transfer dissociation (ETD) plus hybrid versions of these techniques: EThcD and ETciD
- Equipped to perform Real-Time Search (RTS) and Synchronous Precursor Selection (SPS) MS3-based acquisition methods for multiplexed proteomics analyses that incorporate Thermo Scientific Tandem Mass Tags (TMT) reagents for peptide quantitation
- Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
- Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity

Waters Xevo G2-XS q-TOF Mass Spectrometer - with SONAR
Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technology. Capable of multiple mass spectral acquisition options including DDA and DIA modes (MSe and SONAR)