Microbial Analysis, Resources, and Services (MARS)

The Microbial Analysis, Resources and Services (MARS) facility supports research specializing in the analysis of microbial samples and high-throughput processing of nucleic acids. Examples include the characterization of microbiomes, sequencing of small genomes, 96-well and 384-well PCR setup or DNA quantification and other automated liquid handling applications.

Visit Facility Homepage

Contacts

General Contact

mars@uconn.edu
860.486.1417

Staff

Faculty Scientific Advisers

Kumar Venkitanarayanan, professor of animal science and Anup Kollanoor Johny, a postdoctoral fellow in animal science, with chickens at the Poultry Unit on Horsebarn Hill Road on Nov. 29, 2011. (Peter Morenus/UConn Photo)

Location

Campus Address

Engineering and Science Building, Room 309

Mailing Address

ESB Bldg, Room 309
181 Auditorium Rd, Unit 3032
Storrs, CT 06269-3032

Instrumentation

MiSeq

Internal Rates

EpMotion 5075

Liquid handing robot with vacuum and thermometer capabilities

Internal Rates

QIAgility

Liquid handing robot

Internal Rates

QIAxcel

For analyzing nucleic acid fragments

Internal Rates

Biomicrolab XL200 Tube Sorting Robot

Internal Rates

Synergy HT Microplate Reader

Plate Flurometer

Internal Rates

Qubit Microtube Reader

Tube fluorometer

Internal Rates

Odyssey Imaging System

Internal Rates

Covaris M220 Ultra-Sonicator

Can be used by trained individuals independently or by staff for library preparation for the Illumina MiSeq.

384 Well Thermocycler

Services & Rates

Small Genome Sequencing

Isolate sample to data
Isolate DNA to data
Client supplied library to data
Draft genome assembly

Internal Rates

Microbiome and Amplicon Seqeuncing (16S v4, ITS2, etc)

Sample to data
DNA to data
2 step PCR to data
DNA extraction
Data analysis

Internal Rates

$35	Sample to Data	Assisted
$24	DNA to Data	Assisted
$25	2-step PCR Library Prep	Assisted
$18	PCR product with Adapters to Data	Assisted
$11/sample	DNA Isolation	Assisted
$50	Data Analysis, Mothur Standard	Assisted
Unassisted	Users who are trained on the equipment can. purchase aliquots of reagents and consumables to perform their own amplicon processing. Machine time will be billed according to actual usage

Training

For Instrument Access
Workshops and Modules

Internal Rates

External Rates

Custom Data Analysis Consultation

Internal Rates

External Rates

$120/run	50 Cycle, Assisted
$90/run	50 Cycle, Unassisted
$210/run	500 Cycle, Assisted
$180/run	500 Cycle, Unassisted
$300/run	600 Cycle, Assisted
$270/run	600 Cycle, Unassisted

$165	Isolate Sample to Data	BSL1 organisms only
$155	Isolate DNA to Data	No BSL restriction
$120	Client Supplied Library to Data
$50	Genome Data to Draft Genome

$65/hour	Instructional Consulting
$300/participant	2 Day Workshop
$500/participant	5 Day Workshop

$103/hour	Instructional Consulting
$475/participant	2 Day Workshop
$790/participant	5 Day Workshop

Microbial Analysis, Resources, and Services (MARS)

Microbial Analysis, Resources, and Services (MARS)

Contacts

General Contact

Staff

Kendra Maas, Ph.D.

Facility Director

Johanna Cathell

Research Assistant

Faculty Scientific Advisers

Joerg Graf, Ph.D.

Professor

Jonathan Klassen, Ph.D.

Assistant Professor

Kumar Venkitanarayanan, Ph.D.

Professor

Nichole Broderick, Ph.D.

Assistant Professor

Location

Campus Address

Mailing Address

Instrumentation

MiSeq

EpMotion 5075

QIAgility

QIAxcel

Biomicrolab XL200 Tube Sorting Robot

Synergy HT Microplate Reader

Qubit Microtube Reader

Odyssey Imaging System

Covaris M220 Ultra-Sonicator

384 Well Thermocycler

Services & Rates

Small Genome Sequencing

Microbiome and Amplicon Seqeuncing (16S v4, ITS2, etc)

Training

Custom Data Analysis Consultation

$50/hour	Assisted
$20/hour	Unassisted

$50/hour	Assisted
$20/hour	Unassisted

$35/hour	Assisted
$5/hour	Unassisted

$35/hour	Assisted
$5/hour	Unassisted