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Flow Cytometry

Flow Cytometry

Flow Cytometry Facility provides investigators with our expertise and sophisticated instruments for the analysis of cells or particles based on size, internal complexity, and fluorescent signals. Cytometers allow simultaneous analysis of multiple properties of cells or particles and provide information about both single cells and populations. The heterogeneous cells and populations can be identified and quantified. Selected cells or particles can also be physically sorted to obtain pure populations for further analysis or subsequent culture and functional study.

Contacts

Staff

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Amanda DelVichio, Ph.D.

Facility Scientist
Biology Physics Building G05
amanda.delvichio@uconn.edu
860.486.3080

Faculty Scientific Advisers

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Adam Zweifach, Ph.D.

Associate Professor
Adam.zweifach@uconn.edu
860.486.1627
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Andrew Wiemer, Ph.D.

Professor
School of Pharmacy 633
Andrew.wiemer@uconn.edu
860.486.3966
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David Goldhamer, Ph.D.

Professor
Biology Physics Building G24
David.goldhamer@uconn.edu
860.486.8337

Location

Campus Address

Biology Physics Building, Room G05
Storrs Campus

Mailing Address

Flow Cytometry Facility
Center for Open Research Resources and Equipment
91 North Eagleville Road, Unit 3149
Storrs, CT 06269

Instrumentation

Cytek

Cytek Aurora Cell Sorter within BSL2 enclosure

Full Spectrum Sorter: detect the entire emission range of fluorescence molecules as opposed to traditional flow cytometers, where only the peak light emission wavelengths are captured.

Five lasers (UV, violet, blue, yellow-green, and red)

Can detect 64 fluorescent  channels, side and forward scatter, and autofluorescence

Sort up to six populations simultaneously

Tubes and plate sorting available

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BD LSRFortessa X-20 Cell Analyzer

  • Five lasers (355 nm, 405 nm, 488 nm, 561 nm, and 640 nm)
  • Eighteen fluorescence channels plus forward and side scatter
  • High-throughput option allows automated acquisition from 96-well plates
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Analysis Workstation (with FlowJo, Modfit, etc.)

  • Post-acquisition analysis with multiple graphing options
  • Algorithms for analysis and modeling of cell cycle and cell proliferation, etc.
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Reagents and Consumables

  • Common reagents and consumables available to users.

Services & Rates

  • Training and education on flow cytometry theory and facility instruments (required for all new flow users).
  • Un-assisted acquisition (24/7 access to Fortessa Analyzer, and Analysis Stations for independent users)
  • Consultations on experimental design and new strategy development
  • Staff-assisted flow acquisition and data analysis; Cytometric Beads Assay, etc.
  • Project troubleshooting, data interpretation, deep analysis and customized support
  • Software support, Flowjo, Diva, etc.
  • Staff-operated Cell Sorting (Monday-Friday, by appointment through facility director)
  • Contact facility director for consultation, training, sorting schedule, or any other questions.