Metabolic Phenotyping Facility

The Metabolic Phenotyping Facility has capacities to assess cell and mouse bioenergetics, mouse body composition, and blood lipids, cytokines, and others.

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Contacts

Staff

Instrumentation

CLAMS (ABL animal facility 149)

Metabolic cages (Oxymax CLAMS) are used in awake mice to measure energy expenditure, physical activity, indirect calorimetry, and food/water intake. The experiment non-invasively measures O2 consumption and CO2 production in individual mice using metabolic chambers and calculates the respiratory exchange ratio to reflect energy expenditure. Normally, mice are put in a CLAMS metabolic cage for 3 days.

Internal Rates

External Rates

EchoMRI-100 (ABL animal facility 149)

This equipment is a body composition analyzer for small animals (body weight <100 g) that can measure whole body fat, lean, free water, and total water masses in live animals.

Internal Rates

External Rates

Seahorse XFe24 analyzer (ATL 220)

This analyzer determines rates of cellular energy metabolism, e.g., glycolysis and mitochondrial respiration.

The first time user should use “assisted service” for training until he/she can run the assay his/her own.

Internal Rates

External Rates

Luminex MAGPIX for cytokine array (ATL 203)

This equipment is to measure cytokines, chemokines, and hormones in plasma/serum samples.

Internal Rates

$140/plate + cost for a kit/panel	Unassisted	Panel examples: human apolipoprotein panel, human cytokine panel, mouse cytokine panel. The first time user should use “assisted service” for training until he/she can run the assay his/her own.
$200/plate + cost for a kit/panel	Assisted	Panel examples: human apolipoprotein panel, human cytokine panel, mouse cytokine panel. The first time user should use “assisted service” for training until he/she can run the assay his/her own.

External Rates

$189/plate + cost for a kit/panel	Unassisted	Panel examples: human apolipoprotein panel, human cytokine panel, mouse cytokine panel. The first time user should use “assisted service” for training until he/she can run the assay his/her own.
$270/plate + cost for a kit/panel	Assisted	Panel examples: human apolipoprotein panel, human cytokine panel, mouse cytokine panel. The first time user should use “assisted service” for training until he/she can run the assay his/her own.

Cobas clinical chemistry analyzer (Jones 117)

This analyzer measures common clinical chemistry in human and animal blood samples.

Internal Rates

External Rates

Metabolic Phenotyping Facility

Metabolic Phenotyping Facility

Contacts

Staff

Young-Ki Park, Ph.D.

Assistant Research Professor

Instrumentation

CLAMS (ABL animal facility 149)

EchoMRI-100 (ABL animal facility 149)

Seahorse XFe24 analyzer (ATL 220)

Luminex MAGPIX for cytokine array (ATL 203)

Cobas clinical chemistry analyzer (Jones 117)

$200/plate + cost for a cartridge	Unassisted	The first time user should use “assisted service” for training until he/she can run the assay his/her own.
$500/plate + cost for a cartridge	Assisted	The first time user should use “assisted service” for training until he/she can run the assay his/her own.

$675/plate + cost for a cartridge for internal-assisted users	Assisted	The first time user should use “assisted service” for training until he/she can run the array his/her own.
$270/plate + cost for a cartridge	Unassisted	The first time user should use “assisted service” for training until he/she can run the assay his/her own.