Laser Capture Microdissection (LCM) Core

Laser Capture Microdissection (LCM) Core

Laser Capture Microdissection (LCM) is a technique used to lift specifically targeted kind of tissues or cells from a histological section, leaving behind unwanted tissues or cells that could contaminate the molecular purity of subsequent analysis. The UConn Health Laser Capture Microdissection (LCM) Facility house three LCM machines and provides LCM technique training and service to all UConn researchers and external institutions. RNA and DNA can be recovered by dissecting fresh and fixed frozen or paraffin sections for molecular analysis.

Contacts

Staff

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Joel Pachter, Ph.D.

Professor
UConn Health L1021
pachter@nso1.uchc.edu
860.679.3698
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Xi Jiang, M.D.

xijiang@uchc.edu
860.679.2542

Location

Campus Address

Main  Building
Room L1026(L1030)
UConn Health

Mailing Address

263 Farmington Avenue, MC 6022
Farmington CT 06032-6022

Instrumentation

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Arcturus XTTM Laser Microdissection Instrument

IR-Laser and UV Laser Cutting enabled Laser Capture Microdissection (LCM); 2X, 10X and 40X objectives

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ArcturusXTTM Laser Microdissection Instrument

IR-Laser and UV Laser Cutting enabled Laser Capture Microdissection (LCM); 2X, 10X, 20X and 40X objectives. With motorized fluorescence turret and red, green, and blue filter.

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PixCell IIe Laser Capture Microscope with Fluorescence

Services

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Tissue Processing

Tissue isolation, tissue embedding, section, staining

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Laser Capture

Of specific cell types or cellular structures

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Extraction of RNA, DNA, or Protein

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Consultation for Experimental Design

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Grant Preparation Assistance