Induced Pluripotent Stem Cell and Chromosome Core (iPSCC)
Human Genome Editing Services
Noelle Germain, Ph.D.
Faculty Scientific Advisers
Judith Brown, Ph.D.
Assistant Professor in Residence
Rachel O'Neil, Ph.D.
- Beach Hall, Room 323A
Gordon Carmichael, Ph.D.
Cell and Genome Sciences Building
263 Farmington Avenue, MC 6022
Farmington CT 06032-6022
Services & Rates
We provide integration-free reprogramming services using Sendai virus or episomal delivery method. Tissue samples accepted include skin fibroblast, peripheral blood, cord blood, PBMCs, and cells cultured from urine. Reprogramming can be done on feeder containing conditions or feeder independent conditions.
Pluripotency Immunochemistry Test:
- Immunostaining is included in standard iPSC derivation service. However, this service may also be ordered separately from our iPSC derivation service. Markers include Oct4 and Tra-1-60 or SSEA4
- iPSCs are differentiated into embryoid bodies and specification of endoderm, ectoderm, and mesoderm lineages are assayed by qRT-PCR using the Applied Biosystems® TaqMan® hPSC Scorecard.
- We provide a biochemical mycoplasma test using Myco-Alert kit®. Submit 1 - 2 ml of spent culture medium to be tested.
Additional Services and Products
- We will perform QC measures, expand, store, and distribute your custom hESC and iPSC lines when requested by your collaborators or as a back-up storage for your lab.
- Established hESC (H1 and H9, CT1 and CT2) and iPSC lines provided as frozen vials or live cultures
- Validated Serum Replacement (KOSR) to make iPSC/hESC culture medium
- bFGF aliquots, 50 mcg
- We provide training in iPSC/hESC Basic Culture Techniques training as well as troubleshooting support.
- Workshops are available for iPSC/hESC differentiation to neural, cardiomyocyte, and vascular endothelial lineages.
Karyotype analysis of G-banded metaphase chromosomes will detect both numerical and structural chromosome abnormalities. Analysis of human metaphase chromosomes is done using standard protocols for chromosome harvesting, slide-making and G-banding in order to characterize cell lines for chromosome number and rearrangements at a 5-10Mb resolution.
Fluorescence In Situ Hybridization (FISH)
Fluorescence In Situ Hybridization (FISH) will detect the presence/absence or location of a specific gene or chromosome at ~ 100kb resolution. FISH studies are useful to demonstrate micro deletions or duplications and to demonstrate the presence of gene rearrangements. FISH also permits rapid detection of monosomies, trisomies, and numerical sex chromosome abnormalities. FISH involves the hybridization of a target DNA sequence labeled with a fluorescent dye (called a probe).