Induced Pluripotent Stem Cell and Chromosome Core (iPSCC)

Induced Pluripotent Stem Cell and Chromosome Core (iPSCC)

Established in 2006, the University of Connecticut (UConn) Stem Cell Core has substantially contributed to the Connecticut stem cell initiative by providing a central source of technologies and materials for research on human pluripotent stem cells: embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC). The stem cell core is a collaborative effort. Genome Editing, The Cell Bank, and Pluripotent Stem Cell Reprogramming are currently located at the UConn Health campus in Farmington. The Genetics and Genomics Division, including the Chromosome Core, is located at UConn Storrs campus. Single Cell Genomics is based at the Jackson Laboratory for Genomic Medicine in Farmington. The UConn Stem Cell Core is funded by UConn and Connecticut State Stem Cell Research Program. With these crucial services, many Connecticut researchers have developed novel approaches to addressing fundamental biological questions and tackling deleterious diseases.

Contacts

Staff

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Christopher Stoddard

Human Genome Editing Services
stoddard@uchc.edu
860.679.8380
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Noelle Germain, Ph.D.

Lab Manager
germain@uchc.edu
860.679.3828
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Yaling Liu

Research Assistant
yliu@uchc.edu

Faculty Scientific Advisers

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Judith Brown, Ph.D.

Assistant Professor in Residence
Judy.brown@uconn.edu
860.486.6381
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Rachel O'Neil, Ph.D.

Professor
Beach Hall, Room 323A
Rachel.oneil@uconn.edu
860.486.6031
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Gordon Carmichael, Ph.D.

Professor
carmichael@uchc.edu
860.679.2259

Location

Campus Address

Cell and Genome Sciences Building
UConn Health

Mailing Address

263 Farmington Avenue, MC 6022
Farmington CT 06032-6022

Services & Rates

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Reprogramming Services

We provide integration-free reprogramming services using Sendai virus or episomal delivery method.  Tissue samples accepted include skin fibroblast, peripheral blood, cord blood, PBMCs, and cells cultured from urine.  Reprogramming can be done on feeder containing conditions or feeder independent conditions.

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Validation Services

Pluripotency Immunochemistry Test:

  • Immunostaining is included in standard iPSC derivation service. However, this service may also be ordered separately from our iPSC derivation service. Markers include Oct4 and Tra-1-60 or SSEA4
  • iPSCs are differentiated into embryoid bodies and specification of endoderm, ectoderm, and mesoderm lineages are assayed by qRT-PCR using the Applied Biosystems® TaqMan® hPSC Scorecard.
  • We provide a biochemical mycoplasma test using Myco-Alert kit®. Submit 1 - 2 ml of spent culture medium to be tested.
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Additional Services and Products

Biobanking Services:

  • We will perform QC measures, expand, store, and distribute your custom hESC and iPSC lines when requested by your collaborators or as a back-up storage for your lab.
  • Established hESC (H1 and H9, CT1 and CT2) and iPSC lines provided as frozen vials or live cultures
  • Validated Serum Replacement (KOSR)  to make iPSC/hESC culture medium
  • bFGF aliquots, 50 mcg
  • We provide training in iPSC/hESC Basic Culture Techniques training as well as troubleshooting support.
    • Workshops are available for iPSC/hESC differentiation to neural, cardiomyocyte, and vascular endothelial lineages.
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Karyotyping

Karyotype analysis of G-banded metaphase chromosomes will detect both numerical and structural chromosome abnormalities.  Analysis of human metaphase chromosomes is done using standard protocols for chromosome harvesting, slide-making and G-banding in order to characterize cell lines for chromosome number and rearrangements at a 5-10Mb resolution.

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Fluorescence In Situ Hybridization (FISH)

Fluorescence In Situ Hybridization (FISH) will detect the presence/absence or location of a specific gene or chromosome at ~ 100kb resolution. FISH studies are useful to demonstrate micro deletions or duplications and to demonstrate the presence of gene rearrangements. FISH also permits rapid detection of monosomies, trisomies, and numerical sex chromosome abnormalities. FISH involves the hybridization of a target DNA sequence labeled with a fluorescent dye (called a probe).

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Affymetrix CytoScan HD SNP and CNV Analysis