Gel Permeation Chromatography

Gel Permeation Chromatography (GPC) separates a sample based on size. This technique is often used to analyze polymers to understand and predict polymer performance.

Contacts

General Contact

imsinfo@uconn.edu

Staff

Location

Campus Address

Institute of Materials Science

Storrs Campus

Mailing Address

97 North Eagleville Road
Storrs CT 06269-3136

Instrumentation

Waters GPC-1: 1515 HPLC Pump and Waters 717Plus Autoinjector

Provides the molecular weight distribution of polymers fully soluble in toluene.

Specifications

Mobile phase: Tetrahydrofuran (THF)
Column set: Jordi Gel flourinated DVB columns (1-100K, 2-10K & 1-500A). Lower molecular weight column set available.
Injection volume: 10µl – 200µl. Optional 2400 µl sample holding loop available.
Autosampler: Temperature range: 4-40⁰C
Column oven: Four column capacity, temperature range up to 80⁰CSample carousels: 48 vials (4-ml) & 96 vials (1-ml)
Standards: Polystyrene & polymethylmethacrylate
Detectors:
- Waters 2414 Refractive Index Detector (RI): Maximum temperature 50⁰C
- Waters 2487 Dual Absorbance Detector (UV/VIS): Wavelength range 190-700nm. Monitors two wavelengths simultaneously
- Varian 380-LC Evaporative Light Scattering Detector (ELSD): Nebulising gas nitrogen. Temperature range ambient to 90⁰C (nebuliser) & 120⁰C (evaporator)

Agilent 1260 Infinity GPC (System II)

Provides the molecular weight distribution of polymers fully soluble in toluene.

Specifications

Mobile phase: Toluene
Injection volume: Up to 100µl
Sample carousels: 100 vial (1.5-ml)
Column set: Two mixed bed PL columns
Column oven: Three column capacity, temperature range 10-60⁰C
Standards: Polystyrene & polymethylmethacrylate
Detectors:
- Refractive Index Detector (RI): Temperature range ambient plus 5⁰C to 55⁰C
- Variable wavelength Detector: Wavelength range 190-600nm.

Waters GPC-2: Waters 515 HPLC Pump and Waters 717 Autoinjector

Provides the molecular weight distribution of polymers fully soluble in DMAC, NMP or DMF.

Specifications

Mobile phase: Dimethylacetamide (DMAC), methylpyrrolidinone (NMP) or dimethylformamide (DMF)
Column set: Two mixed bed Jordi Gel DVB columns
Injection volume: 10µl – 200µl. Optional 2400 µl sample holding loop available
Autosampler temperature range: ambient to 40⁰C
Column oven: Four column capacity, temperature range up to 80⁰C
Sample carousels: 48 vial (4-ml) & 96 vial (1-ml)
Standards: Polystyrene & polymethylmethacrylate
Detector:
- Waters 2414 Refractive Index Detector (RI). Maximum temperature 50⁰C

Kayeness D4001 Melt Index Tester

Measures ease of flow of a thermoplastic polymer melt. Indirect measure of molecular weight.

Specifications

Gel Permeation Chromatography

Gel Permeation Chromatography

Contacts

General Contact

Staff

Nicholas Eddy, Ph.D.

Research Associate and Lab Manager

Location

Campus Address

Mailing Address

Instrumentation

Waters GPC-1: 1515 HPLC Pump and Waters 717Plus Autoinjector

Agilent 1260 Infinity GPC (System II)

Waters GPC-2: Waters 515 HPLC Pump and Waters 717 Autoinjector

Kayeness D4001 Melt Index Tester