Gordon Carmichael, Ph.D.
Director, Cell and Genome Engineering Core
Professor of Genetics and Genome Sciences
Please contact Yaling Liu firstname.lastname@example.org for quotes and pricing
We provide integration-free reprogramming services using Sendai virus or episomal delivery method. Tissue samples accepted include skin fibroblast, peripheral blood, cord blood, PBMCs, and cells cultured from urine. Reprogramming can be done on feeder containing conditions or
feeder independent conditions.
TALEN, CRISPR and TARGETING
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Custom TALEN Design
- Generation of TALEN and sequencing
- Verification of TALEN
- In-Vitro or In-Vivo expression on exongenous DNA
- In-Vivo expression on genomic locus
Custom CRISPR Design
- CRISPR construction and sequencing
- In-Vitro or In-Vivo on exogenous DNA
- On genomic loci
Vectors for Targeting
- Generation of targeting vector
- Endo-free DNA prep for targeting
- Linearization of targeting vector
- Design and ordering of single stranded ligo for targeting vector
Cell Culture Services
This service can be used to perform cell targeting experiments, selection and genotyping of resistant clones, expansion and freeze of positive cells clones. Cells are targeted by electroporation and plated to perform selection with antibiotics. Number of clones from any targeting experiment can vary widely so fee structure is based upon a per clone basis.
Consultation to discuss a potential project is available at no charge. Following a consultation, a project outline can be developed and a custom quote made for your particular project. There are many custom applications of these technologies that can be used for many experiment types.
Cell and Genome Sciences Building
Cell and Genome Engineering Core
400 Farmington Avenue
Farmington, CT 06030