Center for Genome Innovation (CGI)

Center for Genome Innovation (CGI)

The Center for Genome Innovation (CGI) provides state-of-the-art expertise in genome technologies like Next Generation Sequencing (NGS) and microfluidics platforms for single cell genomic analysis while facilitating genomics research at UConn.

Contacts

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Rachel O'Neill, Ph.D.

Director, Institute for Systems Genomics
Director, Center for Genome Innovation

ESB, Room 305A
rachel.oneill@uconn.edu
860.486.6031

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Bo Reese, Ph.D.

Scientific Director, Center for Genome Innovation
ESB, Room 306E
bo.reese@uconn.edu
860.486.4145
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Lisa LaBelle, M.S.

Research Assistant III
ESB, Room 309
lisa.labelle@uconn.edu
860.486.3618
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Jonnelle Konow, M.S.

Research Assistant III
ESB, Room 309
jonnelle.konow@uconn.edu
860.486-3618
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Kristi Herlth

Research Assistant I
ESB, Room 309
kristi.herlth@uconn.edu
860.486-3618

Instrumentation

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ABI GeneAmp 9700 Gold Block Thermocyclers

Bio-Rad

BioRad Dual Head PCR Thermocyclers

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BioRad CFX96 Real-Time PCR Machine

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Fluidigm BioMark HD

For qPCR and Digital PCR

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Affymetrix Gene Atlas System

Microarray System; WT Expression Array Kit also available

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Affymetrix Gene Chip System

Microarray System; Cytoscan HD Array Kit also available

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ABI 3130 Genetic Analyzer Capillary Sequencer

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Illumina NextSeq 500 NextGen Sequencer

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Illumina MiSeq NextGen Sequencer

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Roche/454 Life Sciences: 454 GS FLX +

DNA Sequencing

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Sample QC

Available instruments include:

  • Agilent 2100 Bioanalyzer Automated Electrophoresis system
  • Agilent 2200 TapeStation Automated Electrophoresis system
  • BioRad Experion Automated Electrophoresis system
  • Thermo-Fisher NanoDrop 2000
  • Agarose gel electrophoresis systems
  • Turner Biosystems Picofluorometer
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Nucleic Acid Fragmentation

Available instruments include:

  • Digilab Hydroshear
  • Qiagen Tissuelyser II
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Microfluidics (Single Cell Genomics)

Available instruments include:

  • Fluidigm C1 Single-Cell Auto Prep
  • Fluidigm Access Array Systems
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Other Ancillary Equipment

  • Beckman Z1 Particle Counter
  • IKA UltraTurrax
  • Allegra X-12R Refrigerated Centrifuge
  • MP Biomedicals FastPrep 24 Sample Prep System
  • Benchmark Plate Shaker

Services & Rates

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Fluidigm Single Cell RNA-Seq on the NextSeq 500

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Laboratory Training

The CGI will hold hands-on, laboratory training for many applications, supported by the Center equipment and staff, including NextGen sequencing and Affymetrix genotyping. Individuals across all UCONN campuses are eligible to participate. All training fees go directly toward instrument maintenance, service contracts and reagents/consumables for training. Participants can bring a sample of their own for training if desired, allowing for participant data collection that can be used as proof of principle or preliminary data for grant submissions.

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Illumina NextSeq 500 Library Preparation

  • TruSeq/EpiGnome Methyl-Seq Library Sample Prep (including Bisulfite conversion)
  • Stranded Total RNA Library Prep
  • Stranded mRNA Library Prep
  • DNA Nano Library Prep (Low Input)
  • Small RNA Library Prep
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GS FLX+ Library Preparation

  • General Rapid Library Prep
  • Paired End Rapid Library Prep (3Kb)
  • cDNA Rapid Library Prep
  • Amplicon Library QC
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Sanger Sequencing and Fragment Analysis

Samples for Sanger Sequencing and STR Fragment analysis can be submitted directly to the CGI (Beach Hall, room 201), or by using the DropBox service.  All submissions must be accompanied by completed online form submission found here.  For more information, see here.

Campus Address

Engineering Science Building
Storrs Campus

UConn Health

Mailing Address

67 North Eagleville Road
Storrs, CT 06269

400 Farmington Avenue
Famington, CT 06030

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Engineering and Science Building, 3rd Floor