Center for Genome Innovation (CGI)

The Center for Genome Innovation (CGI) provides state-of-the-art expertise in genome technologies like Next Generation Sequencing (NGS) and microfluidics platforms for single cell genomic analysis while facilitating genomics research at UConn.

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Contacts

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Instrumentation

ABI GeneAmp 9700 Gold Block Thermocyclers

BioRad Dual Head PCR Thermocyclers

BioRad CFX96 Real-Time PCR Machine

Internal Rates

Fluidigm BioMark HD

For qPCR and Digital PCR

Internal Rates

Affymetrix Gene Atlas System

Microarray System; WT Expression Array Kit also available

Internal Rates

External Rates

Affymetrix Gene Chip System

Microarray System; Cytoscan HD Array Kit also available

Internal Rates

External Rates

ABI 3130 Genetic Analyzer Capillary Sequencer

Internal Rates

Illumina NextSeq 500 NextGen Sequencer

Internal Rates

Illumina MiSeq NextGen Sequencer

Internal Rates

Roche/454 Life Sciences: 454 GS FLX +

DNA Sequencing

Internal Rates

Sample QC

Available instruments include:

Agilent 2100 Bioanalyzer Automated Electrophoresis system
Agilent 2200 TapeStation Automated Electrophoresis system
BioRad Experion Automated Electrophoresis system
Thermo-Fisher NanoDrop 2000
Agarose gel electrophoresis systems
Turner Biosystems Picofluorometer

Internal Rates

Nucleic Acid Fragmentation

Available instruments include:

Digilab Hydroshear
Qiagen Tissuelyser II

Microfluidics (Single Cell Genomics)

Available instruments include:

Fluidigm C1 Single-Cell Auto Prep
Fluidigm Access Array Systems

Internal Rates

Other Ancillary Equipment

Beckman Z1 Particle Counter
IKA UltraTurrax
Allegra X-12R Refrigerated Centrifuge
MP Biomedicals FastPrep 24 Sample Prep System
Benchmark Plate Shaker

Services & Rates

Fluidigm Single Cell RNA-Seq on the NextSeq 500

Laboratory Training

The CGI will hold hands-on, laboratory training for many applications, supported by the Center equipment and staff, including NextGen sequencing and Affymetrix genotyping. Individuals across all UCONN campuses are eligible to participate. All training fees go directly toward instrument maintenance, service contracts and reagents/consumables for training. Participants can bring a sample of their own for training if desired, allowing for participant data collection that can be used as proof of principle or preliminary data for grant submissions.

Internal Rates

Illumina NextSeq 500 Library Preparation

TruSeq/EpiGnome Methyl-Seq Library Sample Prep (including Bisulfite conversion)
Stranded Total RNA Library Prep
Stranded mRNA Library Prep
DNA Nano Library Prep (Low Input)
Small RNA Library Prep

Internal Rates

External Rates

GS FLX+ Library Preparation

General Rapid Library Prep
Paired End Rapid Library Prep (3Kb)
cDNA Rapid Library Prep
Amplicon Library QC

Specifications

Internal Rates

External Rates

Sanger Sequencing and Fragment Analysis

Samples for Sanger Sequencing and STR Fragment analysis can be submitted directly to the CGI (Beach Hall, room 201), or by using the DropBox service. All submissions must be accompanied by completed online form submission found here. For more information, see here.

Internal Rates

$1.00

Run only (no labor)

The customer performs BigDye v3.1 sequencing reactions in his/her own lab using his/her own reagents. The samples are post cleaned and dissolved in 20 μL Hi-Di formamide. Customer will prepare and load samples for sequencing.

$1.60

Run only w/ assistance

The customer performs BigDye v3.1 sequencing reactions in his/her own lab using his/her own reagents. The samples are post cleaned and dissolved in 20 μL Hi-Di formamide. The CGI runs the electrophoresis and delivers data.

$4.00	Full service separate tubes	The customer provides template DNA and primer in separate tubes. The CGI conducts template QC, cycle sequencing reactions, purification, electrophoresis, and data delivery.
$3.30	Full service premixed tubes	The customer provides template DNA and primer in premixed PCR strip tubes according to CGI specifications. The CGI conducts cycle sequencing reactions, purification, electrophoresis, and data delivery.

$104.50	STR Typing	Sample QC and Global Filer Human STR Typing of 21 autosomal Loci, Amelogenin, and (2) Y-chromosome markers for cell line validation (NIH Guidelines). Cost includes STR Report.
$2.20	96-well ABI Sequencing Plate

Campus Address

Engineering Science Building
Storrs Campus

UConn Health

Mailing Address

67 North Eagleville Road
Storrs, CT 06269

400 Farmington Avenue
Famington, CT 06030

Engineering and Science Building, 3rd Floor

$12.00	Single Run (Assisted or unassisted)
$5.97/plate	BioRAD iQ 96-well Real-time PCR Plate
$0.96/seal	BioRad Optical Sealing Tape

$1.00/sample	Unassisted/Assisted Instrument Access
$2.20/plate	96-well Sequencing Plate

$221.00	Unassisted Single Sequencing Run
$233.50	Assisted Single Sequencing Run
$3,914.00	300 Cycle (High Output)
$1,520.00	300 Cycle (Mid Output)
$2,446.25	150 Cycle (High Output)
$950.00	150 Cycle (Mid Output)
$1,273.00	75 Cycle (High Output)

$91.00	Unassisted Single Sequencing Run
$103.50	Assisted Single Sequencing Run
$731.50	Reagent Kit v2 (50 Cycle)
$937.50	Reagent Kit v2 (300 Cycle)
$1,045.00	Reagent Kit v2 (500 Cycle)
$807.50	Reagent Kit v3 (150 Cycle)
$1,410.75	Reagent Kit v3 (600 cycle)

$212.50	Assisted emPCR Workflow
$1,195.00	emPCR Reagent Kit (Multiple samples; One Kit)
$64.60/sample	Additional emPCR Reagents and Consumables
$301.00	Unassisted Single Sequencing Run
$401.00	Assisted Single Sequencing Run
$3,804.39	Sequencing Reagents and Consumables (One Sequencing Run)

Center for Genome Innovation (CGI)

Center for Genome Innovation (CGI)

Contacts

Rachel O'Neill, Ph.D.

Director, Institute for Systems Genomics

Director, Center for Genome Innovation

Bo Reese, Ph.D.

Scientific Director, Center for Genome Innovation

Lisa LaBelle, M.S.

Research Assistant III

Jonnelle Konow, M.S.

Research Assistant III

Kristi Herlth

Research Assistant I

Instrumentation

ABI GeneAmp 9700 Gold Block Thermocyclers

BioRad Dual Head PCR Thermocyclers

BioRad CFX96 Real-Time PCR Machine

Fluidigm BioMark HD

Affymetrix Gene Atlas System

Affymetrix Gene Chip System

ABI 3130 Genetic Analyzer Capillary Sequencer

Illumina NextSeq 500 NextGen Sequencer

Illumina MiSeq NextGen Sequencer

Roche/454 Life Sciences: 454 GS FLX +

Sample QC

Nucleic Acid Fragmentation

Microfluidics (Single Cell Genomics)

Other Ancillary Equipment

Services & Rates

Fluidigm Single Cell RNA-Seq on the NextSeq 500

Laboratory Training

Illumina NextSeq 500 Library Preparation

GS FLX+ Library Preparation

Sanger Sequencing and Fragment Analysis

Campus Address

Mailing Address

$123	Single Run Access Fee (Assisted or Unassisted)
		For detailed rate information, please go here.

$238.00	Single Run (Assisted or Unassisted)
		For detailed rate information, please go here.

$850	Laboratory workshops
$500	Bioinformatics workshops