
Center for Genome Innovation (CGI)
Contacts

Rachel O'Neill, Ph.D.
Director, Institute for Systems Genomics
Director, Center for Genome Innovation
ESB, Room 305A
rachel.oneill@uconn.edu
860.486.6031

Bo Reese, Ph.D.
Scientific Director, Center for Genome Innovation
-
- ESB, Room 306E
- bo.reese@uconn.edu
- 860.486.4145

Lisa LaBelle, M.S.
Research Assistant III
-
- ESB, Room 309
- lisa.labelle@uconn.edu
- 860.486.3618

Jonnelle Konow, M.S.
Research Assistant III
-
- ESB, Room 309
- jonnelle.konow@uconn.edu
- 860.486-3618

Kristi Herlth
Research Assistant I
-
- ESB, Room 309
- kristi.herlth@uconn.edu
- 860.486-3618
Instrumentation

ABI GeneAmp 9700 Gold Block Thermocyclers

BioRad Dual Head PCR Thermocyclers

BioRad CFX96 Real-Time PCR Machine

Fluidigm BioMark HD
For qPCR and Digital PCR

Affymetrix Gene Atlas System
Microarray System; WT Expression Array Kit also available

Affymetrix Gene Chip System
Microarray System; Cytoscan HD Array Kit also available

ABI 3130 Genetic Analyzer Capillary Sequencer

Illumina NextSeq 500 NextGen Sequencer

Illumina MiSeq NextGen Sequencer

Roche/454 Life Sciences: 454 GS FLX +
DNA Sequencing

Sample QC
Available instruments include:
- Agilent 2100 Bioanalyzer Automated Electrophoresis system
- Agilent 2200 TapeStation Automated Electrophoresis system
- BioRad Experion Automated Electrophoresis system
- Thermo-Fisher NanoDrop 2000
- Agarose gel electrophoresis systems
- Turner Biosystems Picofluorometer

Nucleic Acid Fragmentation
Available instruments include:
- Digilab Hydroshear
- Qiagen Tissuelyser II

Microfluidics (Single Cell Genomics)
Available instruments include:
- Fluidigm C1 Single-Cell Auto Prep
- Fluidigm Access Array Systems

Other Ancillary Equipment
- Beckman Z1 Particle Counter
- IKA UltraTurrax
- Allegra X-12R Refrigerated Centrifuge
- MP Biomedicals FastPrep 24 Sample Prep System
- Benchmark Plate Shaker
Services & Rates

Fluidigm Single Cell RNA-Seq on the NextSeq 500

Laboratory Training
The CGI will hold hands-on, laboratory training for many applications, supported by the Center equipment and staff, including NextGen sequencing and Affymetrix genotyping. Individuals across all UCONN campuses are eligible to participate. All training fees go directly toward instrument maintenance, service contracts and reagents/consumables for training. Participants can bring a sample of their own for training if desired, allowing for participant data collection that can be used as proof of principle or preliminary data for grant submissions.

Illumina NextSeq 500 Library Preparation
- TruSeq/EpiGnome Methyl-Seq Library Sample Prep (including Bisulfite conversion)
- Stranded Total RNA Library Prep
- Stranded mRNA Library Prep
- DNA Nano Library Prep (Low Input)
- Small RNA Library Prep

GS FLX+ Library Preparation
- General Rapid Library Prep
- Paired End Rapid Library Prep (3Kb)
- cDNA Rapid Library Prep
- Amplicon Library QC

Sanger Sequencing and Fragment Analysis
Samples for Sanger Sequencing and STR Fragment analysis can be submitted directly to the CGI (Beach Hall, room 201), or by using the DropBox service. All submissions must be accompanied by completed online form submission found here. For more information, see here.
Campus Address
Engineering Science Building
Storrs Campus
UConn Health
Mailing Address
67 North Eagleville Road
Storrs, CT 06269
400 Farmington Avenue
Famington, CT 06030

Engineering and Science Building, 3rd Floor