CCAM Microscopy User Facility
Yi Wu, Ph.D.
Director, CCAM Microscopy User Facility
Associate Professor of Genetics and Genome Sciences
Zeiss LSM 780 Confocal Microscope
This is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's.
Software and Additional Equipment
- Imaris Surpass 3-D rendering and analysis software from Bitplane
- Molecular Devices' MetaMorph; software for analysis, image processing as well as acquisition and device control
- Canon CanoScan 8400F flat bed scanner
- Hitachi's R-1200 Rapid Scan 60Mhz permanent magnet NMR Spectrometer
- Spex Fluorolog single excitation, dual emission spectrofluorimeter
CCAM offers a wide variety of software ranging from applications for analyzing FRAP and other image data to tools for generating models and model parameters, including
- Virtual FRAP Tool
- Microfilament Detector
Zeiss LSM 880
The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. There are two adjacent PMT's and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.
The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen. The LightSheet also has very fast acquisition speeds, allowing you to capture large amounts of data within a fraction of the time compared to other imaging techniques.
Services and Rates
You must be an authorized user, trained by the CCAM staff to use each microscope. There are separate training sessions for each microscope.
Academic Research Building (ARB)
Cell & Genome Sciences Building (CGSB)
Imaging Suite R1510
400 Farmington Avenue
Farmington, CT 06030-6406
263 Farmington Avenue
Farmington, CT 06030