CCAM Microscopy User Facility

CCAM Microscopy User Facility

The Center for Cell Analysis and Modeling (CCAM) Microscopy User Facility provides the UConn research community and other academic and industrial institutes, access to its state-of-the-art equipment for quantitative fluorescence imaging applications. The facility offers access to high end confocal microscopes, one integrated with a two-photon laser, and a light sheet microscope for imaging large, optically cleared samples and for the long-term observation of live biological specimens. CCAM offers its users access to quantitative software, and provides technical support, consultation, and opportunities for collaborative projects.

Contacts

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Yi Wu, Ph.D.

Director, CCAM Microscopy User Facility

Associate Professor of Genetics and Genome Sciences

yiwu@uchc.edu
860.679.1773
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Susan Staurovsky

Lab Manager
staurovsky@uchc.edu
860.679.4686

Instrumentation

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Zeiss LSM 780 Confocal Microscope

This is a combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's.

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Software and Additional Equipment

  • Imaris Surpass 3-D rendering and analysis software from Bitplane
  • Molecular Devices' MetaMorph; software for analysis, image processing as well as acquisition and device control
  • Canon CanoScan 8400F flat bed scanner
  • Hitachi's R-1200 Rapid Scan 60Mhz permanent magnet NMR Spectrometer
  • Spex Fluorolog single excitation, dual emission spectrofluorimeter
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Spex Spectroflurometer

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Software

CCAM offers a wide variety of software ranging from applications for analyzing FRAP and other image data to tools for generating models and model parameters, including

    • Virtual FRAP Tool
    • SpringSaLaD
    • BioNetGen
    • Microfilament Detector
    • SyBil/SBPax
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Zeiss LSM 880

The 880 is mounted on an Axio Observer Z1 with automated xyz control. The system is equipped with a spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging and maximum sensitivity photon counting. There are two adjacent PMT's and a transmitted light detector. The accompanying incubation system, which encloses the microscope, offers stable environmental conditions with temperature, humidity and carbon dioxide control.

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Zeiss LightSheet

The Zeiss LightSheet is an advanced fluorescent microscope that allows you to image optical sections of large, live or fixed samples with virtually no bleaching or phototoxicity to the specimen. The LightSheet also has very fast acquisition speeds, allowing you to capture large amounts of data within a fraction of the time compared to other imaging techniques.

Services and Rates

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Training Session

You must be an authorized user, trained by the CCAM staff to use each microscope. There are separate training sessions for each microscope.

Campus Address

Academic Research Building (ARB)
E6011

Cell & Genome Sciences Building (CGSB)
Imaging Suite R1510
UConn Health

Mailing Address

400 Farmington Avenue
Farmington, CT 06030-6406

263 Farmington Avenue
Farmington, CT 06030