Proteomics & Metabolomics
Contacts
Staff
![pic1 pic1](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/pic1-150x150-circle.jpg)
Jeremy Balsbaugh, Ph.D.
Facility Director
- Office: Pharmacy/Biology Building Room 531
- Lab: Pharmacy/Biology Building Rooms 511/513
- jeremy.balsbaugh@uconn.edu
- 860.486.3319
![TI2gUhuPiT1NOmCvEUKnJgZGi2y4uE4c TI2gUhuPiT1NOmCvEUKnJgZGi2y4uE4c](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/TI2gUhuPiT1NOmCvEUKnJgZGi2y4uE4c-150x150-circle.png)
Jen Liddle, Ph.D.
Facility Scientist
- Office: Pharmacy/Biology Building Room 513
- Lab: Pharmacy/Biology Building Rooms 511/513
- jennifer.liddle@uconn.edu
- 860.486.1591
![DCIM100GOPROG0018108.JPG DCIM100GOPROG0018108.JPG](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/Sonam_3-e1721693009708-150x150-circle.jpg)
Sonam Tamrakar, Ph.D.
Research Assistant III
- Office: Pharmacy/Biology Building Room 511
- Lab: Pharmacy/Biology Building Rooms 511/513
- sonam.tamrakar@uconn.edu
Faculty Scientific Advisers
![spn07002 spn07002](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/spn07002-150x150-circle.png)
Spencer Nyholm, Ph.D.
Associate Professor
- Biology Physics Building 405
- spencer.nyholm@uconn.edu
- 860.486.4886
![das10009 das10009](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/das10009-150x150-circle.jpeg)
Daniel Schwartz, Ph.D.
Associate Professor
- Pharmacy Biology Rm 217/219
- daniel.schwartz@uconn.edu
- 860.486.0596
![xuy04002 xuy04002](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/xuy04002-150x150-circle.png)
![ipingcheng I-Ping Chen DDS, Ph.D. is an associate professor at the UConn School of Dental Medicine and an endodontist at UConn Health. September 25, 2019 (Tina Encarnacion/UConn Health photo)](https://core.uconn.edu/wp-content/uploads/bb-plugin/cache/ipingcheng-circle.jpg)
I-Ping Chen, D.D.S., Ph.D.
Professor
- Oral Health and Diagnostic Science
UConn Health
860.679.1030
Location
Campus Address
Pharmacy/Biology Building, Rooms 511/513
Mailing Address
75 North Eagleville Road, Unit 3149
TLS Room 175
Storrs, CT 06269
Instrumentation
![92vCqeSZk90PZXyWeh7WKhsx53FxOkzB 92vCqeSZk90PZXyWeh7WKhsx53FxOkzB](https://core.uconn.edu/wp-content/uploads/2022/08/92vCqeSZk90PZXyWeh7WKhsx53FxOkzB-300x200.jpg)
Sample Preparation
![Q96Nz19BGEzdcrOEwjs9sDGsanR0ned3 Q96Nz19BGEzdcrOEwjs9sDGsanR0ned3](https://core.uconn.edu/wp-content/uploads/2022/09/Q96Nz19BGEzdcrOEwjs9sDGsanR0ned3-300x225.jpg)
Thermo Scientific Q Exactive HF Mass Spectrometer
- Provides high resolution, accurate mass determinations for MS and MS/MS analysis of proteins and peptides in complex mixtures using High-Field (HF) Orbitrap technology
- Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
- Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity
![pvd425lRpFgebcRZ3YUSpDtb7BWGOH0x pvd425lRpFgebcRZ3YUSpDtb7BWGOH0x](https://core.uconn.edu/wp-content/uploads/2022/09/pvd425lRpFgebcRZ3YUSpDtb7BWGOH0x-225x300.jpg)
Waters Synapt G2-Si Mass Spectrometer
- Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technology
- Capable of multiple mass spectral acquisition options including DDA and DIA modes (MSe)
- Ion mobility technology provides another dimension of separation based on collisional-cross section (analyte size and shape) when mass and retention time aren’t sufficient to separate chemically ambiguous analytes and/or isomers
- Directly coupled to a Waters Acquity UPLC equipped with a PDA detector for high resolution liquid-phase separations at micro-flow rates (μL/min)
![NFoSiBzXBVCd9ua1P7RHXUfYu4k7oVZo NFoSiBzXBVCd9ua1P7RHXUfYu4k7oVZo](https://core.uconn.edu/wp-content/uploads/2022/09/NFoSiBzXBVCd9ua1P7RHXUfYu4k7oVZo-274x300.jpg)
Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer
- Provides multiple mass analysis and detection modes for MS and MS/MS using an Orbitrap or dual-pressure linear ion trap
- Features multiple types of gas-phase fragmentation including collision-induced dissociation (CID), higher energy C-trap dissociation (HCD), electron transfer dissociation (ETD) plus hybrid versions of these techniques: EThcD and ETciD
- Equipped to perform Real-Time Search (RTS) and Synchronous Precursor Selection (SPS) MS3-based acquisition methods for multiplexed proteomics analyses that incorporate Thermo Scientific Tandem Mass Tags (TMT) reagents for peptide quantitation
- Capable of multiple mass spectral acquisition options including data-dependent acquisition (DDA), data-independent acquisition (DIA), and parallel-reaction monitoring (PRM) modes
- Directly coupled to a Thermo Scientific Dionex Ultimate 3000 RSLCnano UPLC for high resolution reversed-phase liquid peptide/protein separations at variable flow regimes, including nanoflow rates for maximum sensitivity
![XIZqdhJv5Q48M7Oc1wvp0AWpX7wd9mBv XIZqdhJv5Q48M7Oc1wvp0AWpX7wd9mBv](https://core.uconn.edu/wp-content/uploads/2022/09/XIZqdhJv5Q48M7Oc1wvp0AWpX7wd9mBv-225x300.jpg)
Waters Xevo G2-XS q-TOF Mass Spectrometer - with SONAR
Provides high resolution, accurate mass measurements for MS and MS/MS analysis of small molecules in complex mixtures using q-TOF technology. Capable of multiple mass spectral acquisition options including DDA and DIA modes (MSe and SONAR)
![LeVDLfOafCcpxZs6lC6lDvOTWif1nwyl LeVDLfOafCcpxZs6lC6lDvOTWif1nwyl](https://core.uconn.edu/wp-content/uploads/2022/08/LeVDLfOafCcpxZs6lC6lDvOTWif1nwyl-150x150.jpg)
Available Software
- Proteomics: MaxQuant (Ref), MaxQuant, Spectronaut v17, Proteome Discoverer v2.5 with XlinkX node (Thermo Scientific), Byonic (Protein Metrics, Inc.), Scaffold v5.0 (Proteome Software, Inc.)
- Metabolomics: Metaboscape (Bruker), Progenesis QI (Nonlinear Dynamics)