Molecular Core Facility

Molecular Core Facility

Molecular Core Facility is a UConn Health Center Research Advisory Committee (HVRAC) supported core. It provides on-site access to a large inventory of cell culture medias, molecular reagents and kits, instrumentation for quantitative PCR, mRNA and cDNA amount and sizes, digital camera systems for photographing and analyzing gels and services such as qPCR setup, animal DNA isolation for genotyping and pcr melting curve analysis. The core also provides throughput logistics for custom oligonucleotide orders, DNAR sequencing and whole plasmid sequencing through contract vendors. Core location BG-004 (around corner from Public safety office at UCONN Health.

Visit Facility Homepage

Contacts

Kevin Claffey, Ph.D.

Director
claffey@uchc.edu
860.679.8713

John Glynn

Manager
molcore@uchc.edu
860.679.1191

Instrumentation

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BIORAD Experion

BioRad CFX96

BIORAD CFX96 with 20 plate autoloader plus analysis software

UCH Covaris

Covaris

UCH Nanodrop

NanoDrop

UCH Qbit

Qbit

Services

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Oligonucleotide Orders

Oligonucleotide orders can be placed through the Molecular Core facility.

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DNA Sequencing

Submission project types include pre-mixed, pre-defined, and custom.

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QPCR Service

The goal of this service is to give the basic science research community access to quantitative PCR in a timely and cost effective manner. The core has the ABI 7900HT and associated software and has accumulated sufficient experience to give direction to the uninitiated investigator in their use.The goal of this service is to give the basic science research community access to quantitative PCR in a timely and cost effective manner. The core has the ABI 7900HT and associated software and has accumulated sufficient experience to give direction to the uninitiated investigator in their use.

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Molecular, Tissue, & Biological Culture Reagents Orders

The Molecular Core Facility in L6094 stocks and places special orders from multiple vendors. The facility has worked with vendors to get no shipping/handling fees and very competative prices for the most commonly used products. There are some specialty items that were bought in bulk to provide users a cost effective product.

The Molecular Core provides a discounted mechanism for ordering both premade and custom probes, as well as common reagents.

Campus Address

Animal Tower (B), Room #BG004
UConn Health Center

Mailing Address

263 Farmington Avenue, MC 6022
Farmington CT 06032-6022