JAX-UConn Single Cell Genomics Center
Contacts
General Contact
For inquiries into using the services of this Center, please send an email to SCBLservice@jax.org.
Staff
Location
Campus Address
Jackson Laboratory for Genomic Medicine
UConn Health Center, Farmington
Mailing Address
10 Discovery Drive
Farmington, CT 06032
Instrumentation
![cIWqNmVFoopZcfrFyZW8pFkCjQ8tudVB cIWqNmVFoopZcfrFyZW8pFkCjQ8tudVB](https://live-cor2e.pantheonsite.io/wp-content/uploads/2022/10/cIWqNmVFoopZcfrFyZW8pFkCjQ8tudVB-225x300.jpeg)
Chromium System (10X Genomics Inc)
A droplet-based single cell RNA-seq system capable of running 8 samples in parallel and generating ~6,000 single cell transcriptomes per sample. More details can be found here.
![qK6Lyqn07uk1JHyQd26n5SlMCBHmspCP qK6Lyqn07uk1JHyQd26n5SlMCBHmspCP](https://live-cor2e.pantheonsite.io/wp-content/uploads/2022/10/qK6Lyqn07uk1JHyQd26n5SlMCBHmspCP-300x283.jpeg)
Drop-Seq Experiment Instrumentation
The lab has all necessary equipment (syringe pumps, microscope) to successfully run Drop-seq experiments as described in Macosko et al. (2015). Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell, 161:1202.
![JxmnItbClFC4KvZ5s3K5sCYCoWl48ImB JxmnItbClFC4KvZ5s3K5sCYCoWl48ImB](https://live-cor2e.pantheonsite.io/wp-content/uploads/2022/10/JxmnItbClFC4KvZ5s3K5sCYCoWl48ImB.jpeg)
Helios™ Mass Cytometer, a CyTOF System (Fluidigm)
Helios™ is the latest generation (3rd) of the CyTOF® mass cytometer system. It is designed to analyze metal-tagged antibodies in cells suspended in solution (similar to a flow cytometer) but can detect up to 35-40 epitopes simultaneously.
![w4Bdvq99mDHH19Lvx45BaskaCuDxyL6e w4Bdvq99mDHH19Lvx45BaskaCuDxyL6e](https://live-cor2e.pantheonsite.io/wp-content/uploads/2022/10/w4Bdvq99mDHH19Lvx45BaskaCuDxyL6e-225x300.jpeg)
Hyperion™ Laser Ablation Module (Fluidigm)
The Hyperion™ is a separate laser ablation module that can be connected to Helios™ to analyze metal-tagged antibodies in tissue. This, in essence, provides a highly multiplexed (up to 37 antibodies) immunohistochemistry platform with a resolution of 1 um. The first publication to describe this approach is Giesen et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11:417.
![mp0HPZRmGSOjzVsHmEKwaMFiOWf3CAD5 mp0HPZRmGSOjzVsHmEKwaMFiOWf3CAD5](https://core.uconn.edu/wp-content/uploads/2022/10/mp0HPZRmGSOjzVsHmEKwaMFiOWf3CAD5-150x150.jpeg)
C1 Single-Cell Auto Prep System (Fluidigm)
A microfluidic chip system that captures individual cells (up to 96 in parallel) and permits automated processing through a series of on-chip molecular steps. Can be used in single cell RNA-seq, single cell ATAC-seq, and other multi-omic approaches.
![hXkd9nFcvsH2oTMjTlQ3NirFY0jstfNJ hXkd9nFcvsH2oTMjTlQ3NirFY0jstfNJ](https://core.uconn.edu/wp-content/uploads/2022/10/hXkd9nFcvsH2oTMjTlQ3NirFY0jstfNJ-300x154.png)
BioMark HD System (Fluidigm)
A high throughput real-time PCR system amenable to use in single cell studies.
![FEC6YYHTbMtwZcpvnD7GoBrL9nxCz5Ya FEC6YYHTbMtwZcpvnD7GoBrL9nxCz5Ya](https://live-cor2e.pantheonsite.io/wp-content/uploads/2022/10/FEC6YYHTbMtwZcpvnD7GoBrL9nxCz5Ya-225x300.jpeg)
ABSL-2 Cell/Tissue Culture
Equipped for cell dissociations on site prior to analysis by single cell approaches.
![2UG4iQernHc9BU7xoXWcg7Uct4RcsZL3 2UG4iQernHc9BU7xoXWcg7Uct4RcsZL3](https://core.uconn.edu/wp-content/uploads/2022/10/2UG4iQernHc9BU7xoXWcg7Uct4RcsZL3-300x225.jpeg)
Echo 525 Liquid Handler (Labcyte)
A liquid handler that moves liquid by sound (acoustic liquid handling) in 25 nl droplets thus greatly reducing the volumes required in various single cell library preparation protocols.