Cell and Genome Engineering Core

Cell and Genome Engineering Core

The University of Connecticut (UConn) Cell and Genome Engineering Core has substantially contributed to Stem Cell Research conducted in Connecticut and beyond by providing a central source of technologies and materials for research on human pluripotent stem cells: embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC). The Genome Editing arm of the Core uses cutting edge genome engineering technologies to develop cellular models.

Contacts

General Contact

Email: ucscicore@uchc.edu

860.679.3828

Gordon Carmichael, Ph.D.

Director

carmichael@uchc.edu

Yaling Liu

Research Assistant
yliu@uchc.edu

Christopher Stoddard

Research Associate
stoddard@uchc.edu

Services

Reprogramming Services

We provide reprogramming service for the following tissue types:

  • Dermal Fibroblasts – as live cell culture or cryopreserved in vial(s)
  • Peripheral Blood
  • Umbilical Cord Blood – frozen

If you would like to discuss the possibility of reprogramming other unique cell types, please contact us.

TALEN, CRISPR and TARGETING

Please contact Christopher Stoddard, stoddard@uchc.edu for quotes and pricing

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Custom TALEN Design

Services include:

  • Generation of TALEN and sequencing
  • Verification of TALEN
    • In-Vitro or In-Vivo expression on exongenous DNA
    • In-Vivo expression on genomic locus
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Custom CRISPR Design

Services include:

  • CRISPR construction and sequencing
  • Validation
    • In-Vitro or In-Vivo on exogenous DNA
    • On genomic loci
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Vectors for Targeting

Services include:

  • Generation of targeting vector
  • Endo-free DNA prep for targeting
  • Linearization of targeting vector
  • Design and ordering of single stranded ligo for targeting vector
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Cell Culture Services

This service can be used to perform cell targeting experiments, selection and genotyping of resistant clones, expansion and freeze of positive cells clones. Cells are targeted by electroporation and plated to perform selection with antibiotics. Number of clones from any targeting experiment can vary widely so fee structure is based upon a per clone basis.

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Consultation

Consultation to discuss a potential project is available at no charge.  Following a consultation, a project outline can be developed and a custom quote made for your particular project.  There are many custom applications of these technologies that can be used for many experiment types.

Campus Address

Cell and Genome Sciences Building
UConn Health

Mailing Address

UConn Health
Cell and Genome Engineering Core
400 Farmington Avenue
Farmington, CT 06030