Central Electron Microscopy Facility (CEMF)

Central Electron Microscopy Facility (CEMF)

The Central Electron Microscope Facility (CEMF) is a UConn Health-supported research facility providing electron microscopy research service for faculty, students, and extramural users. The CEMF has three electron microscopes equipped with digital imaging capabilities available for use by experienced faculty or students.

Contacts

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Stephen King, Ph.D.

Director
sking@uchc.edu
860.679.3347
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Rachael Norris, Ph.D.

Assistant Director
norris@uchc.edu
860.679.2395
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Maya Yankova

Research Associate
yankova@uchc.edu
860.679.2395

Instrumentation

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Hitachi H-7650 Transmission Electron Microscope

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JEOL JSM-5900LV Scanning Electron Microscope

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Zeiss Sigma Gemini HV Field Emission Scanning Electron Microscope

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Sample Preparation Instruments

  • Ultramicrotomes
  • Vacuum Evaporator
  • Sputter Coater
  • Critical Point Dryer
  • High Pressue Freezer
  • Freeze Substitution System
  • Ion Beam Coater

Services and Rates

AMT Camera System
11/30/12
11:08:14 a
700
7.0
80.0
Imaging




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TEM and SEM Specimens

CEMF offers complete processing, examination and photography of TEM (thin-sections) and SEM specimens negative staining.

AMT Camera System
10/23/13
2:49:24 p
80000
7.0
80.0
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Negative Staining of Particulate Samples

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Negative Staining of Particulate Samples

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Other Services

Other services include:

  • Cryosectioning
  • Freeze-fracture
  • Rotary shadow
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Negative Staining of Particulate Samples

Training is available on an individual basis

Policies

Electron Microscopy Facility Policies Concerning Training and EM Use

1. Training of individual students/research staff in the use of the TEM and SEM is performed on an individual basis only.  If multiple researchers from a single lab wish to be trained, each trainee must be scheduled separately.

2. Following initial training in TEM/SEM use, students/research staff may sign up for and use the microscopes.  However, this usage must be overseen by Maya Yankova and so can only occur during normal business hours when Maya Yankova is present in the EM laboratory. Exceptions to this policy are made only in rare cases on an individual basis for highly experienced personnel.

List of Equipment

  • Hitachi H-7650 transmission electron microscope
  • JEOL JSM 5900LV scanning electron microscope (to be decommissioned in September 2023)
  • Zeiss Sigma 360 variable pressure scanning electron microscope with energy dispersion spectroscopy probe (to be installed November 2023)
  • Thermo-Fisher Tundra cryo-electron microscope and auto-grid assembly workstation (to be installed September 2023 in the CGSB, 400 Farmington Ave)
  • Leica EM UC7 ultramicrotome
  • Leica EM UCT ultramicrotome
  • Denton Vacuum Evaporator 502B
  • Denton Vacuum DESK V glow discharge unit
  • Pelco Easiglow glow discharge unit
  • Leica EM CPD030 critical point dryer
  • 1Thermo-Fisher Vitrobot 5 (to be installed September 2023 in the CGSB, 400 Farmington Ave)

Campus Address

UConn Health
B Level: Rooms AB027, AB031

Mailing Address

UConn Health, Main Building
263 Farmington Avenue
Farmington, CT 06030